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Metronidazole and imipenem were administered, and samples of plasma and pleural fluid were collected at predetermined time points. To determine plasma pharmacokinetics of metronidazole and imipenem following administration of a single dose PO (metronidazole, 15 mg/kg) or IV (imipenem, 10 mg/kg) in healthy Thoroughbreds and simulate pleural fluid concentrations following multiple dose administration every 8 hours. However, the present study serves as the basis for future carefully planned clinical trials. Effectiveness of metronidazole administered by IVRLP as a sole therapy against anaerobic infections of synovial structures of the distal limb cannot be determined by a pharmacokinetic study. However, the concentrations rapidly decreased below the minimum inhibitory concentration of potential target pathogens. The serum C max was 1.78 ± 0.93 µg/mL, and the t max was 76 ± 52min.Ĭonclusion Metronidazole administered by IVRLP reached high concentrations in the synovial fluid at 0.5 hours. Only the concentrations of metronidazole at time points 0.25 and 0.5 hours were significantly different (p < 0.001) from synovial concentration before perfusion. The C max in the synovial fluid was 327 ± 208 µg/mL, and the t max was 26 ± 7 minutes.

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Results Four horses were excluded due to low synovial fluid concentrations and not completing the full tourniquet application time. Concentrations of metronidazole were determined by liquid chromatography/tandem mass spectrometry. Blood samples were obtained at the same time points for serum analysis. Synovial fluid samples were collected from the DIPJ before perfusion and at 0.25, 0.5, 2, 12 and 24 hours. Methods Eleven healthy horses had a wide rubber tourniquet applied to the proximal aspect of the antebrachium for 0.5 hours and 500 mg of metronidazole diluted in physiologic saline solution to a total volume of 108 mL was administered by cephalic IVRLP.

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